Accelerating Large Molecule LC-MS Method Development

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Rapid development of sensitive and selective peptide bioanalytical methods are required to support the expanding protein component of pharmaceutical companies’ drug pipeline. Choosing the best MRM for a large molecule peptide or protein is a challenge because multiple charge states are possible, and the many product ion possibilities leave many MRMs to be screened. Manual tuning is tedious, and optimizing for sensitivity via LC injections is time-consuming when monitoring multiple MRMs per peptide, and multiple peptides per protein.

Learning Outcomes

• Learn the advantages of an automated workflow for large molecule characterization and optimization.
• Receive an overview of how to use DiscoveryQuant™ software for peptide tuning and optimization.

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