Determination of Paralytic Shellfish Toxins and Tetrodotoxin in Shellfish using HILIC/MS/MS and MassHunter Software

Separation Science, in collaboration with Agilent, offers an on-demand webinar demonstrating a combined method to ensure the high quality of shellfish, intended for human consumption, using hydrophilic interaction liquid chromatography with tandem mass spectrometry (HILIC/MS/MS) and MassHunter software.

Duration: Approximately 90 minutes

Andrew_Turner_80Andrew D. Turner
(Principal Chemist, Food Safety Group, Cefas Weymouth Laboratory, UK) 

As Principal Chemist in the Cefas Weymouth Food Safety Group, Andrew is responsible for the marine biotoxin testing in shellfish performed on behalf of the UK government competent authorities. He oversees the development and implementation of new methods for food safety surveillance and leads the development of research activities of the chemistry team.



Register for this on-demand learning event ...

Paralytic Shellfish Toxins (PST) are potent neurotoxins produced by several species of phytoplankton, and may bio-accumulate in filter-feeding shellfish. Human consumption of contaminated shellfish can result in Paralytic Shellfish Poisoning (PSP). As a result, monitoring of PST in shellfish is a statutory requirement in many regions of the world, including the EU. However, the detection of PSTs is challenging, due to their highly polar hydrophilic nature and the large number of structurally similar congeners.
Tetrodotoxin (TTX) is another low molecular weight toxin thought to be produced by marine bacteria, which has been found to accumulate in bivalve shellfish. While TTX is not yet stipulated in the EU regulatory requirements, it is known to be as toxic as some analogs of PST.

Monitoring TTX along with other PSTs in shellfish could further protect public health. However, current methodologies used in control labs (including LC/FLD) are not suitable for its detection, and these labs do not have spare resources to add a further test. A method that can detect all PSTs and TTX together in one analytical method would be advantageous for monitoring the risk from all these hydrophilic marine toxins.

In this webinar, the development of a combined method to ensure the high quality of shellfish, intended for human consumption, using hydrophilic interaction liquid chromatography with tandem mass spectrometry (HILIC/MS/MS) and MassHunter software is discussed.

This presentation covers:

  • a discussion of equipment requirements, sample preparation, and method parameters to perform robust and reliable HILIC/MS/MS, and the quality procedures to assure that these requirements are always in place.
  • a method verification exercise along with validated single-laboratory and multi-laboratory method performance characteristics demonstrating that it is suitable for routine analysis.

By viewing this presentation you will learn:

  • how to setup a single method to cover 20 PSTs and TTX using HILIC/MS/MS analysis following a rapid one-step extraction and simple desalting cleanup procedure
  • the performance of the 6495B triple quadrupole LC/MS system under real working conditions
  • how HILIC/MS/MS provides the necessary sensitivity, accuracy, reproducibility, and ruggedness while providing a rapid, simple, and cost‑effective solution in comparison to other analytical detection methods
  • about the results from a recently completed international collaborative validation study for the method, demonstrating acceptable performance across 21 laboratories.

To register for this on-demand webinar simply complete the form on the right. By clicking the 'View on-demand presentation' button, you submit your information to the webinar organizer, who as an independent content provider to analytical and laboratory scientists, will use it to communicate with you regarding this event and other similar platforms run in collaboration with other parties.

Registrants to this webinar are covered by the Eclipse Business Media Privacy Policy.