This presentation is now available to view on-demand. To view, complete the form opposite.
This presentation is now available to view on-demand. To view, complete the form opposite.
High-throughput LC-MS
presented by Martin Gilar
We have evaluated the capability of UPLC systems to perform HT analyses, and found that Acquity UPLC pumps can run 0.25-1 min gradients. When working with sub-minute cycle times, the auto-sampler and data acquisition platform can become a throughput bottleneck. We have evaluated a setup permitting faster LC-UV and LC-MS analyses, using 96- or 384 sample data acquisition in a single file. The performance and peak capacity of HT LC analysis for short, narrow-bore columns is discussed in terms of theoretical and practical performance. The impact of extra-column peak dispersion and peak capacity is evaluated. Attendees to this presentation will learn about the performance of high-throughput LC MS, its achievable speed and performance.
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