---
title: High-throughput LC-MS
description: Hybrid fully porous particles and hybrid-coated solid-core particles, high throughput LC-MS
---

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# High-throughput LC-MS

## ![Developing, Testing and Troubleshooting Chromatography Method Transfer](https://webinar.sepscience.com/hs-fs/hubfs/Webinars/Waters081216/WatersLogo_Reg.png?width=360&name=WatersLogo_Reg.png "Developing, Testing and Troubleshooting Chromatography Method Transfer")

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**High-throughput LC-MS**  
*![martingilar_Web.png](https://webinar.sepscience.com/hs-fs/hubfs/Conferences/Separation%20Science%202017/martingilar_Web.png?width=75&name=martingilar_Web.png "martingilar_Web.png")presented by Martin Gilar*

We have evaluated the capability of UPLC systems to perform HT analyses, and found that Acquity UPLC pumps can run 0.25-1 min gradients. When working with sub-minute cycle times, the auto-sampler and data acquisition platform can become a throughput bottleneck. We have evaluated a setup permitting faster LC-UV and LC-MS analyses, using 96- or 384 sample data acquisition in a single file. The performance and peak capacity of HT LC analysis for short, narrow-bore columns is discussed in terms of theoretical and practical performance. The impact of extra-column peak dispersion and peak capacity is evaluated. Attendees to this presentation will learn about the performance of high-throughput LC MS, its achievable speed and performance. 

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