One Size Does Not Fit All: Considerations for Minimizing Matrix Effect and Maximizing Recovery for Clinical Panels Large and Small

Separation Science, in collaboration with Biotage, offers an on-demand webinar looking at potential issues in LC-MS/MS analysis of large clinical panels, with an emphasis on the impact of matrix components for a variety of biological sample types.

Duration: Approximately 50 minutes

DrWilliamsDr Lee Williams (Biotage GB Limited)
Lee gained a PhD in mass spectrometry under the tutorship of Prof. David Games from the University of Wales, Swansea in 2000. He went on to complete a Post-Doc in the Biochemical Toxicology department at the National Center for Toxicological Research, FDA labs in Arkansas, USA. In 2004 he returned to Wales to join Biotage (Formerly Argonaut) to advance the LC/MS capability within the sample preparation arena. Over the 16 years he has occupied a variety of roles from senior scientist, group leader, lab manager and most recently Director of R&D for Analytical Consumables and Applications.






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LC-MS/MS system sensitivity has moved at an alarming rate over the last 10 years or so. The original perception that due to increased instrument sensitivity sample preparation requirements will decrease has been dispelled. This sensitivity can come at a price, in that levels of contamination not previously detected with less sensitive instruments can now have larger impact on analysis. Combining this with the advent of ultra high-pressure liquid chromatography (UHPLC), the necessity for high-purity components and samples is even more apparent. Given this information the choice of sample preparation technique varies between labs and is generally dependent on several factors: operator familiarity; technique complexity; price per sample; instrument sensitivity; assay sensitivity and limits of quantitation; sample volume; throughput; analyte functionality and target matrix.

This webinar discusses typical clinical matrices such as whole blood, plasma, serum, urine and oral fluid with specific emphasis on a variety of endogenous components. Matrix complexity will be illustrated and related to a variety of clinical assays, demonstrating potential impact on method robustness. Examples will be presented detailing challenges involved for large panel analysis.

By viewing this on-demand presentation you will be provided with:

  • an introduction to individual matrix components for a variety of biological sample types and potential issues in LC/MS analysis
  • consideration of sample preparation technique when dealing with cross-functional analytes in clinical assays.

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