Monitoring Bromethalin Poisoning in Wildlife: Analysis of Adipose Tissue using EMR-Lipid Sorbent

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Speaker

Mike Filigenzi (California Animal Health and Food Safety Laboratory, University of California, Davis, USA)

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Bromethalin is a potent neurotoxic rodenticide. Recent restrictions on the use of second generation anticoagulant rodenticides are likely to result in increased use of bromethalin with subsequent increases of poisonings in non-target species. Post mortem diagnosis of bromethalin poisoning can be accomplished via analysis for desmethylbromethalin, the active metabolite of bromethalin. Results of such testing in our laboratory indicate that adipose tissue is the preferred sample type for this analysis. Separation of desmethylbromethalin from fat is challenging; it is a fairly hydrophobic compound as indicated by its reverse-phase HPLC behaviour.

What does it cover?

The described method utilizes extraction of fat tissue with ethyl acetate and subsequent removal of fat from the extract using Agilent EMR-Lipid sorbent. Results of trials of this method in fat tissue will be discussed as well as its use in diagnosis of bromethalin poisoning in animals.

By attending this webinar you will learn...

• Some important aspects of analysing for bromethalin, a common household rat poison
• Improved cleanup of pure fat samples prior to LC-MS/MS analysis
• Simple workflow for rapid, low-level analysis
• Sub-ppb level testing for a highly lipophilic analyte in a pure fat matrix

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