The applicability of two-dimensional and three-dimensional techniques for effective classification of saffron origin: LC-HRMS and LC-SelexION strategies

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Saffron is one of the oldest and most expensive spices, which is often target of fraudulent activities. In this research, a new strategy of saffron authentication based on metabolic fingerprinting was developed. An extraction procedure was optimized using solid liquid extraction, and a novel detection approach using differential ion mobility separation techniques (SelexION technology) in addition to high resolution LC-MS/MS was developed for analysis. Identification and monitoring of unique metabolite markers made it possible to effectively to identify country of origin for assorted saffron samples. This presentation will show how 2- and 3-dimensional analytical techniques using LC-MS/MS can be effectively applied for food authenticity and fraud monitoring.

By viewing this presentation you will learn...

• how advances in LC-MS/MS technology are accelerating how food can be monitored for fraud and authenticity
• how high resolution LC-MS/MS and SelexION differential ion mobility techniques were uniquely applied for metabolomics profiling of saffron to identify point of origin